Comparative HPTLC fingerprint profile of three types of Kodiveli used in Siddha

Kodiveli is an important Siddha drug used in the formulations such as Podi, Ennai, Thylam, Chithramoola Kuligai and Kudineer. The HPTLC a simple method to differentiate closely related species. profiling of ethanolic extracts roots three species Plumbago (Plumbago zeylanica L. (Venkodiveli), P.indica L., (Senkodiveli) P. auriculata Lam. (Karunkodiveli)) has been carried out using Toluene: Ethyl acetate: Methanol (5:1.5:0.1) solvent system on Silica gel 60 F254-coated aluminium sheets. preliminary qualitative phytochemical screening found same combination phytoconstituents while finger prints have given more refined picture respect number compounds present plumbagin concentration among selected Plumbago. chromatogram peak at Rf. 0.76 common indicating highest all it may be bioactive compound plumbagin. peaks their attributes were different This result will highly useful for precise identification discrimination authentic root materials zeylanica, Indica Auriculata from substitutes adulterants raw market.